Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

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Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label.By extracting DNA from a complete adipose tissue depot stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Book Ucp1-CreERT2 x ROSA26-tdRFP), the number of adipocytes can be determined based on the quantification of the Carvin skimmer parts recombined LoxPRed sites.This highly sensitive system allows for the quantification of white, brown, and brite/beige adipocytes in a spatially unbiased and size-independent manner.For complete details on the use and execution of this protocol, please refer to Moser et al.(2021).